MEK3 Antibody / MAP2K3 / MKK3 (R30446)

MEK3 Antibody / p38 MAP Kinase Kinase Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue using MEK3 Antibody / p38 MAP Kinase Kinase Antibody. MEK3 expression is detected predominantly within the cytoplasm of tumor cells, consistent with its role as a dual-specificity kinase that activates p38 MAP kinases in response to cellular stress, inflammatory cytokines, and environmental stimuli. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.Expected/observed molecular weight ~39 kDa.

MEK3 Antibody / p38 MAP Kinase Kinase Antibody Multi-Species WB. Western blot analysis of 1) human HeLa, 2) human CACO-2, 3) human RT4, 4) rat kidney, 5) mouse kidney, and 6) mouse NIH/3T3 lysates using MEK3 Antibody / p38 MAP Kinase Kinase Antibody. A specific immunoreactive band is detected at approximately 39 kDa in all tested samples, consistent with MEK3 (MAP2K3) expression. The predicted molecular weight of MEK3 is approximately 39 kDa. These results demonstrate reliable detection of MEK3, a dual-specificity protein kinase that phosphorylates and activates p38 MAP kinases in response to cellular stress, inflammatory cytokines, and environmental stimuli, thereby regulating inflammatory signaling, apoptosis, differentiation, and stress-responsive gene expression.