MEK1 Antibody / MAP Kinase Kinase Antibody (R30445)

MEK1 Antibody / MAP Kinase Kinase Antibody Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian cancer tissue using MEK1 Antibody / MAP Kinase Kinase Antibody. MEK1 expression is detected predominantly within the cytoplasm of tumor cells, consistent with its role as a dual-specificity kinase that phosphorylates ERK1 and ERK2 within the RAF-MEK-ERK signaling pathway. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

MEK1 Antibody / MAP Kinase Kinase Antibody A549 Immunofluorescence. Immunofluorescent staining of A549 cells using MEK1 Antibody / MAP Kinase Kinase Antibody (green) with DAPI nuclear counterstain (blue). Enzymatic antigen retrieval was performed for 15 minutes prior to staining. Cells were blocked with 10% goat serum and incubated with the primary antibody (5 ug/ml) overnight at 4°C, followed by a Fluoro488-conjugated goat anti-rabbit IgG secondary antibody. MEK1 displays predominantly cytoplasmic staining, consistent with its role as a dual-specificity kinase that activates ERK1 and ERK2 within the RAF-MEK-ERK signaling pathway to regulate cell proliferation, differentiation, and survival.

MEK1 Antibody / MAP Kinase Kinase Antibody Multi-Species WB. Western blot analysis of 1) human HeLa, 2) human Daudi, 3) rat lung, and 4) rat brain lysates using MEK1 Antibody / MAP Kinase Kinase Antibody. A specific immunoreactive band is detected at approximately 45 kDa in all tested samples, consistent with MEK1 expression. The predicted molecular weight of MEK1 is approximately 43 kDa. These results demonstrate reliable detection of MEK1, a dual-specificity protein kinase that phosphorylates ERK1 and ERK2 within the RAF-MEK-ERK signaling pathway to regulate cell proliferation, differentiation, survival, and cellular responses to extracellular stimuli.