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Home >> Antibodies >> MEK1 Antibody / MAP2K1

MEK1 Antibody / MAP2K1 (F54685)

  Catalog No Formulation Size Price (USD)  
Image F54685-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F54685-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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Western blot testing of mouse cerebellum tissue lysate with MEK1 antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human MDA-MB-435 cell lysate with MEK1 antibody. Predicted molecular weight ~43 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with MEK1 antibody. Predicted molecular weight ~43 kDa.
IHC testing of FFPE mouse stomach tissue with MEK1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P31938
Localization Cytoplasmic, nuclear
Applications Immunohistochemistry (FFPE) : 1:25
Western Blot : 1:500-1:2000
Limitations This MEK1 antibody is available for research use only.
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Description

Map2k1 / Mek1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Learn more about the biological functions, applications, and validated products on our MEK1 Antibody / MAP Kinase Kinase Antibody page.

Application Notes

The stated application concentrations are suggested starting points. Titration of the MEK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 269-296 from the mouse protein was used as the immunogen for the MEK1 antibody.

Storage

Aliquot the MEK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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