MED21 Antibody-Transcription Mediator Complex Antibody V9179

	Catalog No	Formulation	Size	Price (USD)
	V9179-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V9179-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V9179SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

MED21 Antibody Human Glioblastoma Cell IF. Immunofluorescent staining of PFA-fixed human U-87 MG cells using MED21 Antibody (green, clone PCRP-MED21-4B5) with phalloidin cytoskeletal counterstain (red). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates nuclear and cytoplasmic staining in U-87 MG cells, consistent with MED21 localization as a component of the Mediator complex involved in RNA polymerase II transcriptional regulation, chromatin-associated gene expression, and cellular transcription control pathways.

MED21 Antibody HeLa Cell IF. Immunofluorescent staining of PFA-fixed human HeLa cells using MED21 Antibody (green, clone PCRP-MED21-4B5) with phalloidin cytoskeletal counterstain (red). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates nuclear and cytoplasmic staining in HeLa cells, consistent with MED21 function as a Mediator complex subunit involved in RNA polymerase II transcriptional regulation, chromatin-associated gene expression, and control of transcriptional programs.

MED21 Antibody HeLa Cell FACS. Flow cytometric analysis of PFA-fixed human HeLa cells using MED21 Antibody (blue, clone PCRP-MED21-4B5) compared to unstained cells (red). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates a clear positive shift, confirming MED21 detection in HeLa cells and supporting its use for studying Mediator complex function, RNA polymerase II transcriptional regulation, and chromatin-associated gene expression pathways.

MED21 Antibody Human Glioblastoma Cell FACS. Flow cytometric analysis of PFA-fixed human U-87 MG cells using MED21 Antibody (blue, clone PCRP-MED21-4B5) compared to unstained cells (red). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates a positive shift confirming MED21 detection in U-87 MG cells and supporting its use for studying Mediator complex activity, RNA polymerase II transcriptional regulation, and chromatin-associated gene expression pathways.

SDS-PAGE analysis of purified, BSA-free MED21 antibody (clone PCRP-MED21-4B5) as confirmation of integrity and purity.

MED21 Antibody Protein Microarray Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using MED21 Antibody (clone PCRP-MED21-4B5). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates highly specific recognition of MED21 compared to other human proteins on the array. The Z-score indicates the relative antibody binding signal strength, while the S-score measures separation between the intended target and other detected proteins, supporting the specificity of clone PCRP-MED21-4B5 for MED21 detection in transcription regulation and Mediator complex research.

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MED21 Antibody Human Glioblastoma Cell IF. Immunofluorescent staining of PFA-fixed human U-87 MG cells using MED21 Antibody (green, clone PCRP-MED21-4B5) with phalloidin cytoskeletal counterstain (red). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates nuclear and cytoplasmic staining in U-87 MG cells, consistent with MED21 localization as a component of the Mediator complex involved in RNA polymerase II transcriptional regulation, chromatin-associated gene expression, and cellular transcription control pathways.

MED21 Antibody HeLa Cell IF. Immunofluorescent staining of PFA-fixed human HeLa cells using MED21 Antibody (green, clone PCRP-MED21-4B5) with phalloidin cytoskeletal counterstain (red). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates nuclear and cytoplasmic staining in HeLa cells, consistent with MED21 function as a Mediator complex subunit involved in RNA polymerase II transcriptional regulation, chromatin-associated gene expression, and control of transcriptional programs.

MED21 Antibody HeLa Cell FACS. Flow cytometric analysis of PFA-fixed human HeLa cells using MED21 Antibody (blue, clone PCRP-MED21-4B5) compared to unstained cells (red). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates a clear positive shift, confirming MED21 detection in HeLa cells and supporting its use for studying Mediator complex function, RNA polymerase II transcriptional regulation, and chromatin-associated gene expression pathways.

MED21 Antibody Human Glioblastoma Cell FACS. Flow cytometric analysis of PFA-fixed human U-87 MG cells using MED21 Antibody (blue, clone PCRP-MED21-4B5) compared to unstained cells (red). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates a positive shift confirming MED21 detection in U-87 MG cells and supporting its use for studying Mediator complex activity, RNA polymerase II transcriptional regulation, and chromatin-associated gene expression pathways.

SDS-PAGE analysis of purified, BSA-free MED21 antibody (clone PCRP-MED21-4B5) as confirmation of integrity and purity.

MED21 Antibody Protein Microarray Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using MED21 Antibody (clone PCRP-MED21-4B5). MED21 Antibody, a Transcription Mediator Complex Antibody, demonstrates highly specific recognition of MED21 compared to other human proteins on the array. The Z-score indicates the relative antibody binding signal strength, while the S-score measures separation between the intended target and other detected proteins, supporting the specificity of clone PCRP-MED21-4B5 for MED21 detection in transcription regulation and Mediator complex research.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1
Clone Name	PCRP-MED21-4B5
Purity	Protein A/G affinity
UniProt	Q13503
Localization	Nucleus, Cytoplasm
Applications	ELISA (order BSA-free Format For Coating) : Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml
Limitations	This MED21 Antibody / Transcription Mediator Complex Antibody is available for research use only.
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Description

MED21 Antibody recognizes Mediator complex subunit 21 (MED21), a conserved component of the multiprotein Mediator complex that regulates RNA polymerase II-dependent transcription. The Mediator complex acts as a molecular bridge between DNA-bound transcription factors and the basal transcription machinery, allowing regulatory signals from enhancers and promoters to control gene expression. MED21 is part of the Mediator middle module, where it contributes to complex stability, structural organization, and communication between regulatory proteins and RNA polymerase II. MED21 Antibody is widely used to investigate transcriptional regulation, chromatin-associated signaling, and mechanisms controlling gene expression.

The Mediator complex is essential for coordinating diverse transcriptional programs required for development, differentiation, proliferation, and cellular responses to environmental signals. MED21 interacts with other Mediator subunits to support proper assembly and function of the transcriptional regulatory complex. Alterations in Mediator activity have been associated with disrupted gene expression patterns, developmental abnormalities, and cancer biology. As a Transcription Mediator Complex Antibody, MED21 Antibody is valuable for studying transcriptional control pathways and the molecular machinery regulating RNA polymerase II activity.

MED21 has gained research interest because Mediator complex components influence numerous signaling networks, including pathways involved in cell growth, lineage specification, and oncogenic transcription. Changes in Mediator subunit expression or function can affect enhancer-driven gene regulation and transcriptional programs that contribute to disease progression. Investigation of MED21 and related Mediator proteins provides insight into the relationship between chromatin regulation, transcription factor activity, and cellular phenotype.

This mouse monoclonal MED21 Antibody (clone PCRP-MED21-4B5) has been validated by protein microarray analysis, demonstrating specific recognition of MED21 among thousands of full-length human proteins. HuProt(TM) validation provides independent confirmation of antibody specificity, supporting confidence in target recognition. MED21 Antibody, a Transcription Mediator Complex Antibody, is a useful tool for studying Mediator complex biology, RNA polymerase II regulation, chromatin-associated transcription control, and gene expression mechanisms.

Explore additional Epigenetics Antibodies for targets involved in transcriptional regulation, chromatin-associated gene expression, enhancer function, and RNA polymerase II control.

Application Notes

Optimal dilution of the MED21 Antibody / Transcription Mediator Complex Antibody should be determined by the researcher.

Immunogen

Recombinant full-length human MED21 protein was used as the immunogen for the MED21 antibody.

Storage

Aliquot the MED21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

Mediator Complex Subunit 21 Antibody, SRB7 Antibody, SURB7 Antibody, hSrb7 Antibody, Transcription Mediator Subunit Antibody, RNA Polymerase II Regulator Antibody

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MED21 Antibody / Transcription Mediator Complex Antibody [clone PCRP-MED21-4B5] (V9179)

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