- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MED14 Antibody / Mediator Complex Subunit 14 Antibody (R32962)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R32962 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | O60244 |
| Applications | Western Blot : 0.5-1ug/ml Direct ELISA (recombinant Human Protein) : 0.1-0.5ug/ml (BSA-free format available) |
| Limitations | This MED14 Antibody / Mediator Complex Subunit 14 Antibody is available for research use only. |
|
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MED14 Antibody / Mediator Complex Subunit 14 Antibody recognizes MED14, a central structural component of the Mediator complex that is essential for RNA polymerase II-dependent transcription. The Mediator complex functions as a molecular bridge between gene-specific transcription factors and the general transcription machinery, coordinating the activation or repression of thousands of protein-coding genes. MED14 serves as one of the largest scaffold proteins within the complex, helping maintain Mediator architecture while facilitating interactions among multiple subunits and regulatory proteins. Because of its pivotal position within this transcriptional hub, MED14 is required for efficient gene expression across numerous developmental and physiological pathways.
Mediator Complex Subunit 14 plays a critical role in integrating signals from nuclear receptors, sequence-specific transcription factors, and chromatin remodeling proteins. By stabilizing interactions between regulatory proteins and RNA polymerase II, MED14 influences transcription initiation, promoter escape, and productive transcription elongation. The protein participates in signaling pathways controlled by steroid hormone receptors, developmental transcription factors, and stress-responsive regulators, allowing extracellular and intracellular signals to be translated into coordinated changes in gene expression. Loss or disruption of MED14 impairs Mediator complex stability and alters transcriptional programs required for normal cell growth, differentiation, and tissue homeostasis.
MED14 is expressed in a wide variety of tissues and is predominantly localized within the nucleus, where it associates with actively transcribed chromatin. Because the Mediator complex regulates genes involved in proliferation, metabolism, stem cell biology, and differentiation, MED14 has attracted considerable interest in cancer biology and developmental research. Altered MED14 expression or Mediator dysfunction has been associated with several malignancies and inherited developmental disorders, highlighting the importance of Mediator-dependent transcriptional control in both normal physiology and disease. In addition, MED14 contributes to transcriptional responses mediated by hormone receptors and other signaling pathways that govern cellular adaptation to environmental cues.
An MED14 Antibody is a valuable tool for investigating Mediator complex organization, RNA polymerase II-mediated transcription, and transcription factor-dependent gene regulation. Researchers frequently use Mediator Complex Subunit 14 Antibody reagents to examine nuclear localization, transcriptional regulation, chromatin-associated protein complexes, and changes in Mediator function during development, cancer progression, and cellular signaling.
Explore our Transcription Factor Antibodies page for additional proteins involved in transcriptional regulation, Mediator complex function, and RNA polymerase II signaling.
Optimal dilution of the MED14 Antibody / Mediator Complex Subunit 14 Antibody should be determined by the researcher.
A recombinant human protein corresponding to amino acids D181-D375 was used as the immunogen for the MED14 antibody.
After reconstitution, the MED14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
MED14 Antibody, Mediator Complex Subunit 14 Antibody, CRSP2 Antibody, DRIP150 Antibody, TRAP170 Antibody
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