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Home >> Antibodies >> MED13 Antibody

MED13 Antibody (R32960)

  Catalog No Formulation Size Price (USD)  
Image R32960 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) U-2 OS and 2) MCF7 cell lysate with MED13 antibody at 0.5ug/ml. Predicted molecular weight ~239-250 kDa, observed here at ~300 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q9UHV7
Applications Western Blot : 0.5-1ug/ml
Direct ELISA (recombinant human protein) : 0.1-0.5ug/ml (BSA-free format available)
Limitations This MED13 antibody is available for research use only.
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Description

Mediator complex subunit 13 is a protein that in humans is encoded by the MED13 gene. This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Application Notes

Optimal dilution of the MED13 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids L61-Y240 was used as the immunogen for the MED13 antibody.

Storage

After reconstitution, the MED13 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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