ME1 Antibody / Malic enzyme 1 (RQ8885)

Description

ME1 (Malic enzyme 1) is a cytosolic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, producing NADPH in the process. This reaction plays a critical role in cellular metabolism by linking the tricarboxylic acid (TCA) cycle to lipid biosynthesis and antioxidant defense. ME1-derived NADPH is essential for fatty acid and cholesterol synthesis, as well as for maintaining redox balance in cells.

ME1 is expressed in various tissues, with particularly high levels in lipogenic tissues such as liver, adipose tissue, and lactating mammary gland. Its activity is regulated by nutritional and hormonal signals, including carbohydrate intake and insulin, making it a key player in metabolic adaptation. Due to its involvement in energy metabolism and biosynthetic processes, ME1 has been widely studied in the contexts of metabolic regulation and biochemical pathway integration.

The ME1 antibody is a valuable reagent for detecting ME1 in research applications such as western blot, immunohistochemistry, and immunofluorescence. Researchers use the ME1 antibody from NSJ Bioreagents to assess tissue-specific expression, study its role in lipid metabolism, and investigate its contribution to NADPH production. With strong specificity and reproducible performance, the ME1 antibody supports a wide range of studies in cellular metabolism, enzymology, and biochemical research.

Application Notes

Optimal dilution of the ME1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-Q572) was used as the immunogen for the ME1 antibody.

Storage

After reconstitution, the ME1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.