MCMBP Antibody / MCM-binding protein (FY12484)

Immunoprecipitation of MCMBP in 293T whole cell lysate. Western blot analysis of MCMBP using anti-MCMBP antibody. Lane 1: 293T whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-MCMBP antibody in 293T whole cell lysate, Lane 3: anti-MCMBP antibody (2ug) + 293T whole cell lysate (500ug). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCMBP antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The expected molecular weight of MCMBP is ~73 kDa.

Western blot analysis of MCMBP using anti-MCMBP antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCMBP antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of MCMBP is ~73 kDa.