MCM7 Antibody for IF / Minichromosome Maintenance Complex Component 7 Antibody (R31674)

MCM7 Antibody for IF - immunofluorescence of human colon cancer tissue. FFPE human colon cancer tissue was stained with MCM7 Antibody for IF using a rabbit polyclonal antibody targeting Minichromosome maintenance complex component 7 / MCM7. Strong nuclear fluorescence is observed in many carcinoma epithelial cells, consistent with the nuclear localization of this DNA replication licensing factor and the high proliferative activity characteristic of colon cancer. Antigen retrieval was performed by steaming tissue sections in pH6 citrate buffer for 20 minutes prior to staining.

MCM7 Antibody for IF - immunofluorescence staining of human colon cancer tissue. FFPE human colon carcinoma tissue was stained with MCM7 Antibody for IF using a rabbit polyclonal antibody targeting Minichromosome maintenance complex component 7 / MCM7. Green nuclear fluorescence is observed in many tumor epithelial cells lining the glandular structures, consistent with the nuclear localization of this DNA replication licensing factor in proliferating cells. The staining highlights clusters of actively cycling carcinoma cells within the tumor epithelium. Heat-induced epitope retrieval was performed by steaming tissue sections in pH6 citrate buffer for 20 minutes prior to staining.

MCM7 Antibody for IF - immunofluorescence staining of human U-2 OS cells. FFPE human U-2 OS cells were stained with MCM7 Antibody for IF using a rabbit polyclonal antibody targeting Minichromosome maintenance complex component 7 / MCM7 (green). The signal localizes predominantly to cell nuclei, consistent with the known nuclear role of this DNA replication licensing factor. DAPI nuclear counterstain highlights cell nuclei (blue), demonstrating strong overlap with MCM7 staining in actively proliferating cells. Heat-induced epitope retrieval was performed by steaming sections in pH6 citrate buffer for 20 minutes prior to staining.

Immunocytochemical staining of FFPE human A549 cells with MCM7 antibody. HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human lung cancer tissue with MCM7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse testis tissue with MCM7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat intestinal tissue with MCM7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human HeLa, 2) human K562, 3) human Jurkat, 4) human MCF7, 5) rat C6 and 6) mouse Neuro-2a cell lysate with MCM7 antibody. Expected molecular weight: 80-90 kDa.

Flow cytometry testing of human A431 cells with MCM7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM7 antibody.

Flow cytometry testing of human U937 cells with MCM7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM7 antibody.