MCM7 Antibody Rabbit Polyclonal / Minichromosome Maintenance Protein 7 Antibody (R30890)

MCM7 Antibody Rabbit Polyclonal - immunofluorescence staining of human MCF7 cells. FFPE human MCF7 cells were stained with MCM7 Antibody Rabbit Polyclonal to detect Minichromosome maintenance protein 7 / MCM7 (green). Nuclear fluorescence is observed in many cells, consistent with the known nuclear localization of this DNA replication licensing factor in proliferating cells. Alpha Tubulin monoclonal antibody staining highlights the cytoskeletal microtubule network (red), providing cellular context for the nuclear MCM7 signal. Heat-induced epitope retrieval was performed by steaming sections in pH6 citrate buffer for 20 minutes prior to staining.

IHC staining of FFPE human ovarian serous adenocarcinoma tissue with MCM7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

MCM7 Antibody Rabbit Polyclonal - immunohistochemistry of human rectal adenocarcinoma tissue. FFPE human moderately differentiated rectal adenocarcinoma tissue was stained with MCM7 Antibody Rabbit Polyclonal to detect Minichromosome maintenance protein 7 / MCM7. HRP-DAB brown chromogenic signal highlights predominantly nuclear staining in tumor epithelial cells within glandular gland structures, consistent with the nuclear localization of this DNA replication licensing factor in proliferating carcinoma cells. Hematoxylin counterstain marks cell nuclei (blue). Heat-induced epitope retrieval was performed by boiling tissue sections in pH8 EDTA buffer for 20 minutes and allowing sections to cool prior to staining.

IHC staining of FFPE human bladder cancer tissue with MCM7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) HeLa and 20 MCF7 cell lysate using rabbit polyclonal MCM7 antibody. Expected molecular weight: 80~90 kDa.

Flow cytometry testing of human HL60 cells with MCM7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM7 antibody.