MCM7 Antibody Protein Microarray Validated MCM7/1469 / Minichromosome Maintenance Protein 7 Antibody [clone MCM7/1469] (V3502)

Description

Minichromosome maintenance protein 7 (MCM7) is a nuclear DNA replication factor encoded by the MCM7 gene and functions as an essential component of the minichromosome maintenance helicase complex responsible for eukaryotic DNA replication. MCM7 Antibody Protein Microarray Validated MCM7/1469 recognizes this replication licensing protein and supports research focused on DNA synthesis, cell cycle progression, and proliferative signaling in normal and malignant cells.

MCM7 is a member of the highly conserved MCM protein family that forms the MCM2-7 helicase complex, a ring-shaped molecular machine responsible for unwinding double-stranded DNA at replication forks. During the G1 phase of the cell cycle, MCM complexes are loaded onto chromatin at replication origins as part of the pre-replication complex. Activation of this helicase during S phase enables DNA strand separation and allows DNA polymerases and associated replication machinery to copy the genome with high fidelity.

MCM7 antibody, also referred to as CDC47 antibody or P1-MCM3 antibody in the literature, detects a nuclear protein strongly associated with actively proliferating cells. The protein localizes predominantly to chromatin within the nucleus where it participates in replication origin licensing and helicase activation. Because MCM7 expression correlates with cell cycle activity, the protein is commonly used as a marker of proliferating cells in studies of tissue growth, regeneration, and tumor biology.

Structurally, MCM7 contains conserved ATP-binding and ATP-hydrolysis motifs characteristic of the AAA+ ATPase family. These domains provide the energy required for helicase activity, driving conformational changes that separate DNA strands and enable replication fork progression. Tight regulation of MCM7 and other MCM proteins ensures that DNA replication occurs only once per cell cycle, helping maintain genomic stability and preventing aberrant DNA duplication.

Elevated expression of MCM7 has been reported in numerous cancers including colorectal carcinoma, breast cancer, lung cancer, and other rapidly proliferating malignancies. Increased levels of MCM proteins reflect the heightened DNA replication activity present in tumor cells and have made the complex widely studied in cancer biology and cell cycle research. A mouse monoclonal antibody such as MCM7 Antibody Protein Microarray Validated MCM7/1469 provides a useful tool for detecting Minichromosome maintenance protein 7 expression and studying mechanisms that regulate DNA replication and cellular proliferation.

Application Notes

Titering of the MCM7 Antibody Protein Microarray Validated MCM7/1469 may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody.

Storage

Store the MCM7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

CDC47 antibody, P1-MCM3 antibody, DNA replication licensing factor MCM7 antibody, Minichromosome maintenance protein 7 antibody, MCM7 replication factor antibody