- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MCM7 Antibody for WB / Minichromosome Maintenance Protein 7 Antibody [clone MCM7/1466] (V3365)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V3365-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V3365-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V3365SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | MCM7/1466 |
| Purity | Protein G affinity chromatography |
| UniProt | P33993 |
| Localization | Nuclear |
| Applications | Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This MCM7 antibody is available for research use only. |
|
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Minichromosome maintenance protein 7 (MCM7) is a nuclear DNA replication factor encoded by the MCM7 gene and functions as a key component of the minichromosome maintenance helicase complex responsible for eukaryotic DNA replication. The MCM7 Antibody for WB (clone MCM7/1466) is designed for western blot detection of this replication licensing protein and supports analysis of MCM7 expression levels in cell lysates and experimental models investigating DNA replication and cell cycle regulation.
Western blot analysis of MCM7 typically reveals a band in the ~80-90 kDa range corresponding to the full-length Minichromosome maintenance protein 7. Because MCM7 is a chromatin-associated replication factor, the protein is commonly detected in nuclear or whole cell lysates derived from proliferating cell populations. In SDS-PAGE immunoblot experiments, MCM7 may appear as a slightly broadened band or doublet due to phosphorylation state differences or other post-translational modifications that occur during cell cycle progression.
MCM7 antibody, also referred to as CDC47 antibody and P1-MCM3 antibody in the literature, detects a replication licensing protein that is highly expressed in actively dividing cells. The protein forms part of the heterohexameric MCM2-7 helicase complex that unwinds double-stranded DNA at replication forks during S phase. During the G1 phase of the cell cycle, MCM complexes assemble at replication origins as part of the pre-replication complex, licensing chromatin for DNA synthesis. Activation of the helicase during S phase enables DNA strand separation and allows DNA polymerases to replicate the genome.
Structurally, MCM7 contains conserved ATP-binding and ATP-hydrolysis motifs characteristic of the AAA+ ATPase family of helicases. These domains supply the energy required for helicase activity and drive conformational changes within the MCM complex that enable DNA strand separation and replication fork progression. Proper regulation of MCM proteins ensures that chromosomal DNA is replicated only once during each cell cycle, maintaining genomic stability and preventing aberrant DNA duplication.
Western blot detection of MCM7 is widely used in studies examining cell proliferation, DNA replication dynamics, and replication licensing mechanisms. Elevated expression of MCM7 has been reported in numerous cancers including colorectal carcinoma, breast cancer, and lung cancer, reflecting the increased DNA replication activity present in rapidly proliferating tumor cells. A mouse monoclonal antibody such as MCM7 Antibody for WB (clone MCM7/1466) provides a reliable tool for detecting Minichromosome maintenance protein 7 by immunoblot analysis and studying molecular pathways that regulate DNA replication and cell cycle progression.
The stated application concentrations are suggested starting amounts. Titration of the MCM7 Antibody for WB may be required due to differences in protocols and secondary/substrate sensitivity.
Amino acids 195-319 were used as the immunogen for the MCM7 antibody.
Store the MCM7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
CDC47 antibody, P1-MCM3 antibody, DNA replication licensing factor MCM7 antibody, Minichromosome maintenance protein 7 antibody, MCM7 replication factor antibody
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