MCM5 Antibody / MCM complex protein 5 [clone MCM5/3200] (V5943)

Western Blot analysis of human Ramos cell lysate using MCM5/MCM complex protein 5 antibody (clone MCM5/3200). Expected molecular weight: 80~90 kDa.

Immunohistochemistry analysis of MCM5/MCM complex protein 5 antibody (clone MCM5/3200) in human tonsil. Formalin-fixed, paraffin-embedded human tonsil shows strong nuclear brown staining in proliferating lymphoid cells within germinal centers, consistent with MCM complex protein 5 expression in actively cycling B cells, while surrounding mantle zone lymphocytes demonstrate reduced staining intensity. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of non-specific nuclear staining. Heat-induced epitope retrieval was performed by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95oC followed by cooling at room temperature for 20 minutes prior to staining.

Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM5/MCM complex protein 5 antibody (clone MCM5/3200). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.