MCM5 Antibody / MCM complex protein 5 [clone 4G10] (RQ6284)

Immunohistochemistry analysis of MCM5/MCM complex protein 5 antibody (clone 4G10) in human gallbladder adenocarcinoma. Formalin-fixed, paraffin-embedded human gallbladder adenocarcinoma shows strong nuclear brown staining in tumor epithelial cells on the right panel, consistent with MCM5 as a DNA replication licensing factor and proliferation-associated marker, while the left panel processed without primary antibody demonstrates absence of specific nuclear staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

IHC staining of FFPE human lung cancer with (right) and without (left) MCM5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pancreatic cancer with MCM5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human A431 cells with MCM5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human MCF-7, 2) rat thymus, 3) rat RH35 and 4) mouse thymus lysate with MCM5 antibody. Expected molecular weight: 80~90 kDa.

Flow cytometry testing of human A549 cells with MCM5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM5 antibody.