MCM4 Antibody / DNA replication licensing factor 4 [clone MCM4/3041] (V5941)

Immunohistochemistry staining of formalin-fixed, paraffin-embedded human colon with MCM4/DNA replication licensing factor 4 antibody (clone MCM4/3041). Strong nuclear brown staining is observed in proliferating epithelial cells lining the colonic glands, consistent with MCM4 as a replication licensing factor and proliferation-associated marker, while surrounding stromal cells show minimal to weak labeling. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10mM Tris with 1mM EDTA for 20 min followed by cooling prior to staining.

Western blot analysis of MCM4/DNA replication licensing factor 4 antibody (clone MCM4/3041) in human A431 cell lysate. A prominent band is detected at approximately 100 kDa, corresponding to the predicted molecular weight of MCM4, with a characteristic doublet pattern consistent with different phosphorylation states of this cell cycle–regulated protein.

Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM4/DNA replication licensing factor 4 antibody (MCM4/3041). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.