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Home >> Antibodies >> MCM3 Antibody

MCM3 Antibody (R30735)

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  Catalog No Formulation Size Price (USD)  
Image R30735 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with MCM3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with MCM3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MCM3 antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MCM3 antibody testing of rat intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
ICC testing of HeLa cells
IHC-P: MCM3 antibody testing of mouse intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MCM3 antibody testing of rat intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa and 2) Jurkat cell lysate. Expected molecular weight: 90~115 kDa.
Western blot testing of mouse 1) NIH-3T3 and 2) HEPA1-6 cell lysate. Expected molecular weight: 90~115 kDa.
Flow cytometry testing of human SiHa cells with MCM3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM3 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P25205
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml (1)
Immunocytochemistry : 0.5-1ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This MCM3 antibody is available for research use only.
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Description

Minichromosome maintenance, S. cerevisiae, homolog of, 3, also called RLFB or P1 Protein, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the MCM3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. HIER: steamed with pH6 citrate buffer is required for FFPE testing.

Immunogen

An amino acid sequence from the N-terminus of human MCM3 (REAQRDYLDFLDDEE) was used as the immunogen for this MCM3 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the MCM3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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