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Home >> Antibodies >> MCM3 Antibody / Minichromosome maintenance complex component 3

MCM3 Antibody / Minichromosome maintenance complex component 3 [clone MSVA-503M] (V5940)

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  Catalog No Formulation Size Price (USD)  
Image V5940-100UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 544.50
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V5940-20UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 259
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DNA replication licensing factor MCM3 Mouse Monoclonal Antibody (MSVA-503M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas. <br><br>Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
DNA replication licensing factor MCM3 Mouse Monoclonal Antibody (MSVA-503M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MSVA-503M
UniProt P25205
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This MCM3/Minichromosome maintenance complex component 3 antibody is available for research use only.
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Application Notes

1. Optimal dilution of the MCM3/Minichromosome maintenance complex component 3 antibody should be determined by the researcher.

2. This MCM3/Minichromosome maintenance complex component 3 antibody is recombinantly produced by expression in CHO cells.

Immunogen

A recombinant fragment (aa 650-750) of human MCM3 protein (exact sequence is proprietary) was used as the immuongen for the MCM3/Minichromosome maintenance complex component 3 antibody.

Storage

MCM3/Minichromosome maintenance complex component 3 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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