- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MCM3 Antibody / Minichromosome maintenance complex component 3 [clone MCM3/6706] (V5119)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V5119-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V5119-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V5119SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | MCM3/6706 |
| Purity | Protein A/G affinity |
| UniProt | P25205 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This MCM3 antibody is available for research use only. |
|
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MCM3 antibody (clone MCM3/6706) targets Minichromosome maintenance complex component 3, a nuclear protein that functions as a DNA replication licensing factor and essential regulator of cell cycle progression. The MCM3 gene encodes one of six core subunits of the MCM2-7 complex, which assembles at replication origins to form the replicative helicase required for unwinding double-stranded DNA. Because MCM3 is loaded onto chromatin during early G1 and remains present throughout the active phases of the cell cycle, MCM3 antibody serves as a robust marker of replication-competent cells.
Minichromosome maintenance complex component 3 plays a critical role in establishing the pre-replication complex, ensuring that DNA replication occurs once per cell cycle. Following origin licensing, the MCM2-7 complex cooperates with CDC45 and the GINS complex to form the CMG helicase, the central enzymatic machinery driving replication fork progression. Nuclear localization of MCM3 reflects its function in chromatin-associated replication control. In immunohistochemical analysis, MCM3 antibody typically produces distinct nuclear staining in actively dividing epithelial, lymphoid, and progenitor cell populations, while differentiated or quiescent cells demonstrate minimal signal.
In oncologic research, elevated MCM3 expression has been reported across multiple tumor types including colorectal carcinoma, breast carcinoma, lung adenocarcinoma, prostate carcinoma, and various hematologic malignancies. Expanded nuclear staining patterns frequently correlate with increased proliferative fraction and disrupted cell cycle regulation. Because MCM3 identifies cells licensed for replication, it may detect a broader proliferative compartment than markers limited to mitotic activity or DNA synthesis alone. This property makes MCM3 antibody useful in tumor grading studies, evaluation of dysplastic lesions, and investigation of growth kinetics in neoplastic tissues.
Beyond cancer biology, MCM3 is fundamental to normal tissue renewal and stem or progenitor cell maintenance. Tight regulation of replication licensing prevents re-replication and preserves genomic stability. Aberrant expression or deregulated activation of the MCM complex can contribute to replication stress and genomic instability, processes closely linked to malignant transformation. MCM3 antibody (clone MCM3/6706) is designed to detect nuclear Minichromosome maintenance complex component 3 in research applications, supporting studies of DNA replication control, cell cycle regulation, proliferative capacity, and replication licensing mechanisms in both normal and disease contexts.
Optimal dilution of the MCM3 antibody should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 650-750) from the human protein was used as the immunogen for the MCM3 antibody.
Aliquot the MCM3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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