MCM3 Antibody / Cell Cycle Marker [clone MCM3/3221] (V5118)

Immunohistochemistry analysis of recombinant MCM3/Cell Cycle Marker antibody (clone MCM3/3221) in FFPE human colon carcinoma tissue. Strong nuclear brown staining is observed in malignant epithelial cells, consistent with the role of MCM3 as a DNA replication licensing factor and proliferation marker. Adjacent stromal cells show comparatively lower labeling. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10mM Tris with 1mM EDTA for 20 min followed by cooling prior to staining.

IHC staining of FFPE human tonsil tissue with recombinant MCM3/Cell Cycle Marker antibody (clone MCM3/3221). Inset: PBS instead of primary antibody, secondary only control.

SDS-PAGE analysis of purified, BSA-free recombinant MCM3/Cell Cycle Marker antibody (clone MCM3/3221) as confirmation of integrity and purity.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant MCM3/Cell Cycle Marker antibody (clone MCM3/3221). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.