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Home >> Antibodies >> MCM2 Antibody

MCM2 Antibody (R30734)

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  Catalog No Formulation Size Price (USD)  
Image R30734 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of MCM2 antibody and human lysate 1: SW620; 2: PANC; 3: Jurkat; 4: HeLa. Predicted molecular weight: 100~130 kDa.
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Western blot testing of MCM2 antibody and human lysate 1: SW620; 2: PANC; 3: Jurkat; 4: HeLa. Predicted molecular weight: 100~130 kDa.
IHC-P: MCM2 antibody testing of human intestinal cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MCM2 antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IF/ICC staining of A549 cells with MCM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P49736
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This MCM2 antibody is available for research use only.
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Description

Minichromosome maintenance, S. cerevisiae, homolog of, 2, also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in two crucial steps of the cell cycle, namely, onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the MCM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human MCM2 (NKFSHDLKRKMILQQF) was used as the immunogen for this MCM2 antibody.

Storage

After reconstitution, the MCM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

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   2.   View all MCM2 antibodies

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