MCEMP1 Antibody / Mast cell-expressed membrane protein 1 (FY13113)

Western blot analysis of MCEMP1 using anti-MCEMP1 antibody. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCEMP1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. MCEMP1 antibody detects a single band at ~28 kDa across the indicated samples. Although the calculated mass is ~21 kDa, MCEMP1 is a glycosylated, single-pass membrane protein that commonly migrates at ~27-30 kDa on SDS-PAGE.