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Home >> Antibodies >> MCCC1 Antibody / 3-Methylcrotonyl-CoA carboxylase 1

MCCC1 Antibody / 3-Methylcrotonyl-CoA carboxylase 1 (RQ8598)

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  Catalog No Formulation Size Price (USD)  
Image RQ8598 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Western blot testing of 1) human SiHa, 2) human RT4 and 3) rat liver tissue lysate with MCCC1 antibody. Predicted molecular weight ~80 kDa.
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Western blot testing of 1) human SiHa, 2) human RT4 and 3) rat liver tissue lysate with MCCC1 antibody. Predicted molecular weight ~80 kDa.
Western blot testing of 1) human HepG2, 2) human Caco-2, 3) human A431, 4) human HaCaT, 5) mouse liver and 6) rat liver tissue lysate with MCCC1 antibody. Predicted molecular weight ~80 kDa.
Flow cytometry testing of fixed human MCF7 cells with MCCC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCCC1 antibody.
Availability 1-3 days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q96RQ3
Localization Cytoplasm
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This MCCC1 antibody is available for research use only.
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Description

This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Application Notes

Optimal dilution of the MCCC1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids Q104-E715) was used as the immunogen for the MCCC1 antibody.

Storage

After reconstitution, the MCCC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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