MCAM Antibody 4C12 / MUC18 Antibody [clone 4C12] (RQ5661)

MCAM Antibody 4C12 by IHC in Human Rectal Cancer Tissue. Immunohistochemistry staining of FFPE human rectal cancer tissue using mouse monoclonal MCAM antibody 4C12. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling. Brown immunoreactivity is observed in scattered vascular and stromal structures, with prominent staining along the walls of small blood vessels. Much of the surrounding glandular epithelium exhibits comparatively weak staining. The distribution is consistent with expression of MUC18/CD146 in vascular-associated cells.

MCAM Antibody 4C12 by IHC in Human Breast Cancer Tissue. Immunohistochemistry staining of FFPE human breast cancer tissue using mouse monoclonal MCAM antibody 4C12. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling. Strong brown immunoreactivity outlines vascular structures within the tumor-associated stroma, while much of the surrounding tissue exhibits relatively weak staining. This pattern is consistent with the expression of MUC18/CD146 in vascular endothelial and associated cells.

MCAM Antibody 4C12 by IHC in Human Placenta Tissue. Immunohistochemistry staining of FFPE human placenta tissue using mouse monoclonal MCAM antibody 4C12. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling. Strong brown immunoreactivity is observed along numerous vascular and villous structures, with prominent staining outlining vessel walls and selected cellular boundaries. The distribution is consistent with MUC18/CD146 expression in placental vascular-associated cells.

MCAM Antibody 4C12 by IHC in Frozen Human Placenta Tissue. Immunohistochemistry staining of frozen human placenta tissue using mouse monoclonal MCAM antibody 4C12. Strong brown immunoreactivity outlines numerous vascular structures, with prominent staining along vessel walls and selected cell membranes. The surrounding tissue exhibits comparatively weaker staining. This experiment demonstrates detection of MUC18/CD146 in frozen placental tissue, complementing the FFPE validation.

MCAM Antibody 4C12 by WB in Human A375 and HeLa Cell Lysates. Western blot analysis of human A375 melanoma cells (lane 1) and HeLa cervical cancer cells (lane 2) using mouse monoclonal MCAM antibody 4C12. A strong, distinct immunoreactive band is detected at approximately 130 kDa in both samples, consistent with the higher apparent molecular weight of extensively glycosylated MUC18/CD146. The mature protein commonly migrates above its predicted polypeptide mass because of N-linked glycosylation. Minimal additional immunoreactivity is visible in either lane.

MCAM Antibody 4C12 by FACS in Human SiHa Cells. Flow cytometry analysis of human SiHa cervical cancer cells using mouse monoclonal MCAM antibody 4C12 at 1 ug per million cells following blocking with goat serum. The histogram compares antibody-stained cells (blue), isotype control (green), and unstained cells (red). The blue population exhibits a clear rightward fluorescence shift relative to the green isotype control, demonstrating detection of cell surface MUC18/CD146. The isotype control also shows increased fluorescence relative to unstained cells, indicating some background signal under the assay conditions.