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Home >> Antibodies >> MBP Antibody / Myelin Basic Protein

MBP Antibody / Myelin Basic Protein (R30229)

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Image R30229 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of MBP antibody and mouse brain tissue lysate. Multiple isoforms may be visualized from 20~37 kDa.
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Western blot testing of MBP antibody and mouse brain tissue lysate. Multiple isoforms may be visualized from 20~37 kDa.
IHC-P: MBP antibody testing of rat brain tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE mouse brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of MBP antibody with 1) mouse brain and 2) rat brain lysate. Multiple isoforms may be visualized from 20~37 kDa.
Immunofluorescent staining of FFPE rat brain with MBP antibody (red), anti-GFAP mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MBP antibody testing of mouse hippocampus tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of mouse cerebral cortex tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of mouse brain tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of mouse cerebellum tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of rat hippocampus tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of rat cerebral cortex tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of rat cerebellum tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P02686
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Limitations This MBP antibody is available for research use only.
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Description

Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the MBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids KLGGRDSRSGSPMARR were used as the immunogen for this MBP antibody.

Storage

After reconstitution, the MBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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