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Home >> Antibodies >> MBP Antibody / Myelin basic protein

MBP Antibody / Myelin basic protein [clone rMBP/9431] (V5763)

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  Catalog No Formulation Size Price (USD)  
Image V5763-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V5763-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V5763SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recmousemono
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Immunohistochemistry analysis of recombinant MBP / Myelin basic protein antibody (clone rMBP/9431) in human brain tissue. Formalin-fixed, paraffin-embedded human brain tissue was stained using recombinant MBP antibody (clone rMBP/9431). Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 20 minutes followed by cooling prior to antibody incubation. Strong cytoplasmic and fiber-like staining is observed throughout white matter regions, highlighting myelinated axonal tracts, while neuronal cell bodies demonstrate minimal staining. The inset image represents a secondary-only negative control using PBS in place of primary antibody and shows absence of specific immunoreactivity.
Western blot testing of human brain tissue lysate with recombinant MBP antibody (clone rMBP/9431). Predicted molecular weight: ~22 kDa, ~33 kDa (two isoforms).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rMBP/9431
Purity Protein G affinity
UniProt P02686
Localization Cell membrane, Myelin membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This MBP antibody is available for research use only.
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Description

MBP Antibody recognizes Myelin basic protein, a major structural component of the myelin sheath in the central nervous system. Myelin basic protein antibody is widely used in neuroscience research as a marker of myelinating oligodendrocytes and compact myelin. Encoded by the MBP gene, Myelin basic protein plays a critical role in myelin membrane compaction and stabilization of the multilayered myelin sheath surrounding axons.

Myelin basic protein is localized to the cytoplasmic face of the myelin membrane and is highly expressed in oligodendrocytes in the central nervous system and in Schwann cells in the peripheral nervous system. MBP antibody is commonly used to evaluate white matter integrity, axonal insulation, and oligodendrocyte differentiation. The protein contributes to adhesion of cytoplasmic leaflets within myelin, enabling formation of dense compact myelin necessary for rapid nerve conduction.

Alterations in Myelin basic protein expression have been associated with demyelinating diseases such as multiple sclerosis, leukodystrophies, traumatic brain injury, and other neuroinflammatory conditions. Decreased MBP expression may reflect myelin loss, while abnormal distribution can indicate active demyelination or remyelination processes. For this reason, MBP Antibody is frequently applied in studies of neurodegeneration, central nervous system injury, and therapeutic remyelination strategies.

Myelin basic protein exists in multiple alternatively spliced isoforms, with the 18.5 kDa isoform being one of the most studied components of compact myelin. Post-translational modifications including phosphorylation and deimination influence its structural properties and interaction with lipid membranes. MBP Antibody provides a reliable tool for assessing myelin structure, oligodendrocyte biology, and white matter pathology in both normal and disease contexts.

Application Notes

Optimal dilution of the MBP antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-100 from human Myelin basic protein was used as the immunogen for the MBP antibody.

Storage

Aliquot the MBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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