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Home >> Antibodies >> MBP Antibody / Myelin Basic Protein

MBP Antibody / Myelin Basic Protein (R30229)

  Catalog No Formulation Size Price (USD)  
Image R30229 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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MBP Antibody in Mouse Brain WB. Western blot testing of mouse brain tissue lysate with MBP Antibody / Brain Myelin Marker. Multiple MBP isoforms are detected at approximately 20-37 kDa. This MBP Antibody demonstrates detection of myelin basic protein isoforms in mouse brain tissue.
MBP Antibody Detection in Rat Brain IHC. Immunohistochemistry staining of rat brain tissue with MBP Antibody / Brain Myelin Marker, HRP secondary antibody and TSA 570 reagent. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 10-20 min followed by cooling before testing. The staining demonstrates MBP distribution within rat brain tissue.
MBP Antibody Myelin Staining in Mouse Brain IF. Immunofluorescence staining of FFPE mouse brain tissue with MBP Antibody / Brain Myelin Marker (green) at 2 ug/ml and DAPI nuclear stain (blue). HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 min. The observed staining highlights MBP-positive myelinated structures in mouse brain.
MBP Antibody Visualization in Rat Brain IF. Immunofluorescence staining of FFPE rat brain tissue with MBP Antibody / Brain Myelin Marker (green) at 2 ug/ml with DAPI nuclear counterstain (blue). Tissue sections underwent HIER by steaming in pH 6 citrate buffer for 20 min. MBP staining reveals the distribution of brain myelin in rat neural tissue.
MBP Antibody Detection of Rat Brain Myelin IF. Immunofluorescence staining of FFPE rat brain with MBP Antibody / Brain Myelin Marker (green) at 2 ug/ml and DAPI (blue). HIER was performed in pH 6 citrate buffer by steaming for 20 min. The staining pattern demonstrates the organization of MBP-positive myelinated structures.
MBP Antibody Myelin Distribution in Rat Brain IF. Immunofluorescence staining of FFPE rat brain tissue with MBP Antibody / Brain Myelin Marker (green) at 2 ug/ml and DAPI nuclear stain (blue). Tissue sections were steamed in pH 6 citrate buffer for 20 min for antigen retrieval. This image demonstrates MBP localization and myelin architecture in rat brain.
MBP Antibody in Rat and Mouse Brain WB. Western blot testing of rat brain (lane 1) and mouse brain (lane 2) lysates with MBP Antibody / Brain Myelin Marker. Multiple MBP isoforms are detected at approximately 20-37 kDa in both species. This MBP Antibody demonstrates detection of myelin basic protein in rat and mouse brain lysates.
MBP Antibody and GFAP Dual Staining in Rat Brain IF. Immunofluorescence staining of FFPE rat brain tissue with MBP Antibody / Brain Myelin Marker (red), anti-GFAP monoclonal antibody (green) and DAPI nuclear stain (blue). HIER was performed by steaming sections in pH 6 citrate buffer for 20 min. Dual labeling visualizes MBP-positive myelin together with GFAP-positive astrocytic structures in rat brain.
MBP Antibody in Mouse Hippocampus IHC. Immunohistochemistry staining of FFPE mouse hippocampus tissue with MBP Antibody / Brain Myelin Marker. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 10-20 min followed by cooling before testing. MBP staining demonstrates myelin distribution within the mouse hippocampus.
MBP Antibody in Mouse Cerebral Cortex IHC. Immunohistochemistry staining of FFPE mouse cerebral cortex tissue with MBP Antibody / Brain Myelin Marker. Tissue sections underwent HIER by boiling in pH 8 EDTA buffer for 10-20 min and were allowed to cool before testing. The observed staining highlights MBP expression within myelinated structures of the cerebral cortex.
MBP Antibody Brain Myelin Staining in Mouse IHC. Immunohistochemistry staining of FFPE mouse brain tissue with MBP Antibody / Brain Myelin Marker. HIER was performed in pH 8 EDTA buffer by boiling for 10-20 min followed by cooling. This MBP Antibody demonstrates the distribution of myelin basic protein in mouse brain.
MBP Antibody in Mouse Cerebellum IHC. Immunohistochemistry staining of FFPE mouse cerebellum tissue with MBP Antibody / Brain Myelin Marker. Tissue sections were boiled in pH 8 EDTA buffer for 10-20 min and allowed to cool before testing. MBP staining reveals myelinated structures within the mouse cerebellum.
MBP Antibody in Rat Hippocampus IHC. Immunohistochemistry staining of FFPE rat hippocampus tissue with MBP Antibody / Brain Myelin Marker. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 10-20 min followed by cooling. The staining demonstrates regional MBP distribution within rat hippocampal tissue.
MBP Antibody in Rat Cerebral Cortex IHC. Immunohistochemistry staining of FFPE rat cerebral cortex tissue with MBP Antibody / Brain Myelin Marker. HIER was performed in pH 8 EDTA buffer for 10-20 min by boiling followed by cooling before testing. MBP-positive structures illustrate myelin distribution throughout the rat cerebral cortex.
MBP Antibody in Rat Cerebellum IHC. Immunohistochemistry staining of FFPE rat cerebellum tissue with MBP Antibody / Brain Myelin Marker. Tissue sections underwent HIER by boiling in pH 8 EDTA buffer for 10-20 min followed by cooling before testing. The staining highlights MBP-associated myelin architecture within rat cerebellar tissue.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P02686
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Limitations This MBP antibody is available for research use only.
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Description

MBP Antibody / Brain Myelin Marker detects myelin basic protein (MBP), a major structural component of the myelin sheath surrounding neuronal axons. Encoded by the MBP gene, myelin basic protein contributes to the formation, compaction and stability of myelin membranes. In the central nervous system, MBP is produced primarily by oligodendrocytes and is widely used as a brain myelin marker for investigating myelination, glial cell development and nervous system organization.

MBP is a highly basic protein that associates with the cytoplasmic surfaces of myelin membranes. Its interactions with membrane lipids help bring adjacent layers together, producing the compact multilamellar structure required for normal myelin function. Alternative splicing of the MBP gene generates several protein isoforms that differ in molecular weight and developmental expression. These isoforms contribute to myelin assembly and maturation and can be detected as multiple bands when brain tissue is analyzed by Western blot.

As a brain myelin marker, MBP is commonly examined in the hippocampus, cerebral cortex, cerebellum and other regions of the central nervous system. Its expression provides a useful indicator of myelin distribution and can be studied alongside glial markers such as GFAP to investigate the relationship between myelinated structures and astrocytes. MBP is also relevant to studies of oligodendrocyte differentiation, developmental myelination and axon-glia interactions.

Changes in MBP expression, distribution or integrity are investigated in models of demyelination and remyelination, including research related to multiple sclerosis and experimental autoimmune encephalomyelitis. MBP is a recognized myelin-associated autoantigen and is also studied in neurological injury and neurodegenerative conditions involving changes to white matter or myelin integrity.

This rabbit polyclonal MBP Antibody has been validated by Western blot, immunohistochemistry and immunofluorescence using mouse and rat brain samples. Validation includes hippocampus, cerebral cortex and cerebellum, as well as dual immunofluorescence detection of MBP and GFAP in rat brain. NSJ Bioreagents supplies antibodies for neuroscience and glial cell research. An MBP Antibody can be used to investigate brain myelin distribution, oligodendrocyte biology, nervous system development and mechanisms associated with myelin damage and repair.

For broader research on myelin structure and MBP expression, see our Myelin Basic Protein Antibody / CNS Myelin Marker page.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the MBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids KLGGRDSRSGSPMARR were used as the immunogen for this MBP antibody.

Storage

After reconstitution, the MBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

MBP antibody, Myelin Basic Protein antibody, Brain Myelin Marker antibody, Myelin A1 Protein antibody

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