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Home >> Antibodies >> MBP Antibody / Myelin basic protein

MBP Antibody / Myelin basic protein [clone MSVA-390R] (V5939)

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  Catalog No Formulation Size Price (USD)  
Image V5939-100UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 559
Image
V5939-20UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 259
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Myelin basic protein Rabbit Recombinant Monoclonal Antibody (MSVA-390R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas. <br><br>Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Myelin basic protein Rabbit Recombinant Monoclonal Antibody (MSVA-390R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-390R
UniProt P02686
Localization Myelin membrane, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This MBP/Myelin basic protein antibody is available for research use only.
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Application Notes

1. Optimal dilution of the MBP/Myelin basic protein antibody should be determined by the researcher.

2. This MBP/Myelin basic protein antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragment (around amino acids 150-250) of human MBP (exact sequence is proprietary) was used as the immuongen for the MBP/Myelin basic protein antibody.

Storage

MBP/Myelin basic protein antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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