MASPIN Antibody / SERPINB5 [clone SERPINB5/4978] (V4579)

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using MASPIN antibody (clone SERPINB5/4978). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Immunohistochemistry analysis of MASPIN / SERPINB5 antibody in human skin. FFPE human skin tissue was stained with MASPIN / SERPINB5 antibody (clone SERPINB5/4978). HRP-DAB brown chromogenic signal is observed predominantly within the epidermis, showing strong cytoplasmic staining in keratinocytes with focal nuclear positivity. The staining is most prominent in the basal and suprabasal layers of the epidermis, consistent with epithelial expression of MASPIN. The dermal compartment shows minimal background staining. The inset negative control, in which PBS was used in place of the primary antibody, shows no specific brown staining. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes followed by cooling prior to antibody incubation.