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Home >> Antibodies >> MASPIN Antibody / SERPINB5

MASPIN Antibody / SERPINB5 [clone 7G4E1] (RQ6922)

  Catalog No Formulation Size Price (USD)  
Image RQ6922 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human tonsil tissue with MASPIN antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MASPIN antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HaCaT and 3) Caco-2 cell lysate with MASPIN antibody. Predicted molecular weight: 42~45 kDa.
Flow cytometry testing of human SiHa cells with MASPIN antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MASPIN antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 7G4E1
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P36952
Applications Western blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This MASPIN antibody is available for research use only.
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Description

SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.

Application Notes

Optimal dilution of the MASPIN antibody should be determined by the researcher.

Immunogen

Recombinant human MASPIN protein (amino acids M1-A350) was used as the immunogen for the MASPIN antibody.

Storage

After reconstitution, the MASPIN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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