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Home >> Antibodies >> MART-1 Antibody Cocktail

MART-1 Antibody Cocktail [clone A103 + M2-7C10 + M2-9E3] (V2118)

  Catalog No Formulation Size Price (USD)  
Image V2118-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V2118-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V2118SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
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V2118IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 459
IHC testing in human melanoma stained with MART-1 antibody cocktail.
IHC testing of FFPE testis tissue with MART-1 antibody cocktail.
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1 (A103) + IgG2b (M2-7C10 & M2-9E3)
Clone Name A103 + M2-7C10 + M2-9E3
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 2315
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This MART-1 antibody cocktail is available for research use only.
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Description

This antibody cocktail recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody cocktail does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein (A103; M2-7C10; M2-9E3) was used as the immunogen for this MART-1 antibody cocktail.

Storage

Store the MART-1 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA, MART-1 antibody, Melan-A antibody

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