- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MART-1 Antibody / Melanoma antigen recognized by T-cells 1 / MLANA [clone MSVA-900M] (V5893)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V5893-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 559 | |
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V5893-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 259 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | MSVA-900M |
| UniProt | Q16655 |
| Localization | Endoplasmic reticulum membrane, Golgi apparatus, Melanosome, trans-Golgi network membrane |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 |
| Limitations | This MART-1/Melanoma antigen recognized by T-cells 1 antibody is available for research use only. |
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MART-1 antibody detects Melanoma antigen recognized by T-cells 1, a melanocyte differentiation antigen encoded by the MLANA gene and localized predominantly to the cytoplasm and melanosome membranes of melanocytes. Melanoma antigen recognized by T-cells 1 is commonly referred to as MART-1 and plays an essential role in melanosome structure, melanocyte differentiation, and antigen presentation within the melanocytic lineage. The MART-1 antibody is widely used in research and diagnostic pathology to study melanocyte biology and to identify melanocytic lesions based on its restricted expression profile.
Melanoma antigen recognized by T-cells 1 functions as a melanosomal membrane-associated protein that stabilizes gp100 and supports proper melanosome maturation. Expression is largely confined to normal melanocytes, retinal pigment epithelial cells, and melanocytic tumors, making MART-1 antibody a valuable reagent for distinguishing melanocytic cells from non-melanocytic lineages. Because MLANA expression is preserved in the majority of melanomas, this antibody is frequently used as a marker of melanocytic differentiation in tissue-based studies.
In immunohistochemistry applications, MART-1 antibody typically produces cytoplasmic brown chromogenic staining in melanocytes and melanoma cells, while most epithelial, mesenchymal, and hematopoietic tissues remain negative. This selective expression pattern supports its use in evaluating melanocytic lesions, studying melanoma progression, and investigating melanocyte-associated pathways. Clone MSVA-900M is designed to recognize Melanoma antigen recognized by T-cells 1 in research applications and provides consistent detection of MLANA-positive cells in formalin-fixed, paraffin-embedded tissues when appropriate antigen retrieval conditions are applied.
Because of its lineage specificity, MART-1 antibody is also useful in comparative studies alongside other melanocytic markers such as SOX10, PMEL, and S100 family proteins, enabling refined characterization of melanocytic tumors and differentiation states. Its restricted expression profile continues to make Melanoma antigen recognized by T-cells 1 a cornerstone marker in melanocyte and melanoma research.
1. Optimal dilution of the MART-1/Melanoma antigen recognized by T-cells 1 antibody should be determined by the researcher.
2. This MART-1/Melanoma antigen recognized by T-cells 1 antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Recombinant full-length hMART-1 protein was used as the immunogen for the MART-1/Melanoma antigen recognized by T-cells 1 antibody.
MART-1/Melanoma antigen recognized by T-cells 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
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