MART-1 Antibody [clone M2-7C10] (V2116)

Description

MART-1 Antibody Clone M2-7C10 recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Explore our Melan-A Antibody / Melanocyte Lineage Marker clone A103 page for additional validation data and research applications involving melanoma biology, melanocytic tumor identification, and pigment cell differentiation pathways.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MART-1 Antibody Clone M2-7C10 to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human MART-1 protein was used as the immunogen for this antibody.

Storage

Store the MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A antibody, MLANA antibody, MART-1 antibody, Melan-A antibody