MARCOL Antibody / Myristoylated alanine-rich C kinase substrate-like (FY12235)

Western blot analysis of MARCOL using anti-MARCOL antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human SIHA whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCOL antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for MARCOL at approximately 30 kDa. The expected band size for MARCOL is at 30 kDa.