MAPKAPK2 Antibody / p38 MAPK Stress Signaling Marker (R36061)

MAPKAPK2 Antibody Knockout Rescue WB. Western blot analysis of mouse embryonic fibroblast lysates using goat polyclonal MAPKAPK2 antibody (Cat. # R36061). Lysates from double knockout cells are shown in lanes 1 and 3, while lane 2 contains knockout cells rescued by reintroduction of the MK2 / MAPKAPK2 gene. A distinct band is restored at approximately 45 kDa in rescued cells, consistent with the predicted molecular weight of MAP kinase-activated protein kinase 2, a downstream kinase within the p38 stress-response signaling pathway.

MAPKAPK2 Antibody Overexpression Specificity WB. Western blot analysis of HEK293 cell lysates overexpressing mouse MK2-GFP or MK5-GFP using goat polyclonal MAPKAPK2 antibodies Cat. # R36061 (left) and Cat. # R35113 (right). Both antibodies detect a prominent band at approximately 72 kDa in MK2-GFP-expressing lysates, consistent with the predicted molecular weight of MAP kinase-activated protein kinase 2 fused to GFP. Minimal cross-reactivity with the related MK5-GFP kinase construct supports selective recognition of MAPKAPK2 / MK2.

MAPKAPK2 Antibody Immunoprecipitation. Immunoprecipitation analysis using goat polyclonal MAPKAPK2 antibodies Cat. # R36061 and Cat. # R35113 (1.5 ug each) was performed on lysates from MK2/MK3 double knockout mouse embryonic fibroblasts with and without rescued MK2 expression following retroviral transduction. Immunoblot detection with an unrelated rabbit polyclonal antibody identifies a band at approximately 45 kDa selectively in rescued samples, consistent with the predicted molecular weight of MAP kinase-activated protein kinase 2 / MK2 and supporting specific immunoprecipitation of endogenous MAPKAPK2.