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Home >> Antibodies >> MAPK8/9 Antibody

MAPK8/9 Antibody (R30478)

  Catalog No Formulation Size Price (USD)  
Image R30478 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of MAPK8/9 antibody and Lane 1: rat brain; 2: rat thymus and human 3: MCF-7; 4: HeLa; 5: Jurkat; 6: MM231; 7: CEM cell lysate.
IHC-P: MAPK8/9 antibody testing of rat intestine tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human intestinal cancer with MAPK8/9 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P45983
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This MAPK8/9 antibody is available for research use only.
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Description

Mitogen-activated protein kinase 8 is an enzyme that in humans is encoded by the MAPK8 gene. The protein encoded by this gene is a member of the MAP kinase family. The International Radiation Hybrid Mapping Consortium mapped the MAPK8 gene to chromosome 10. The single long open reading frame encodes a putative protein kinase with a predicted mass of 44.2 kD. This kinase play a key role in T cell proliferation, apoptosis and differentiation.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the MAPK8/9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the middle region of human MAPK8/9 (EREHTIEEWKELIYKEVMDL) was used as the immunogen for this MAPK8/9 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the MAPK8/9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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