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Home >> Antibodies >> MAPK6 Antibody

MAPK6 Antibody (R32252)

  Catalog No Formulation Size Price (USD)  
Image R32252 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat brain, 2) rat skeletal muscle, 3) human PANC and 4) mouse NIH3T3 lysate with MAPK6 antibody. Predicted molecular weight ~83 kDa but routinely observed at ~97 kDa.
IHC testing of FFPE human lung cancer with MAPK6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q16659
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This MAPK6 antibody is available for research use only.
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Description

Mitogen-activated protein kinase 6, or p97 MAPK, is an enzyme that in humans is encoded by the MAPK6 gene. It is mapped to 15q21. The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblastsupon treatment with serum or phorbol esters.

Application Notes

Optimal dilution of the MAPK6 antibody should be determined by the researcher.

Immunogen

Amino acids 520-721 of human MAPK6 were used as the immunogen for the MAPK6 antibody.

Storage

After reconstitution, the MAPK6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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