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Home >> Antibodies >> MAP6D1 Antibody / MAP6 domain-containing protein 1

MAP6D1 Antibody / MAP6 domain-containing protein 1 (RQ8444)

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  Catalog No Formulation Size Price (USD)  
Image RQ8444 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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IHC staining of FFPE rat brain tissue with MAP6D1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain tissue with MAP6D1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with MAP6D1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioma tissue with MAP6D1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human T-47D, 2) rat brain and 3) mouse brain tissue with MAP6D1 antibody. Predicted molecular weight ~21 kDa.
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with MAP6D1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MAP6D1 antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with MAP6D1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9H9H5
Localization Cytoplasm
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This MAP6D1 antibody is available for research use only.
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Description

This gene encodes a protein highly similar to the mouse MAP6 domain containing 1 protein, which is related to the STOP proteins. Based on the study of the mouse protein, the encoded protein may function as a calmodulin-regulated neuronal protein that binds and stabilizes microtubules but also associates with the Golgi membranes through N-terminal palmitoylation.

Application Notes

Optimal dilution of the MAP6D1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (M1-V199) was used as the immunogen for the MAP6D1 antibody.

Storage

After reconstitution, the MAP6D1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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