Mammaglobin Antibody - HuProt Validated / SCGB2A2 Antibody [clone MGB/4056] (V8616)

Mammaglobin Antibody - HuProt Validated immunohistochemistry of human breast carcinoma. FFPE human breast carcinoma tissue was stained with Mammaglobin Antibody - HuProt Validated (clone MGB/4056). HRP-DAB brown chromogenic signal highlights cytoplasmic staining in clusters of tumor epithelial cells within glandular structures, consistent with expression of Mammaglobin A / SCGB2A2 in breast carcinoma cells, while surrounding stromal cells show little to no staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.

HC staining of FFPE human breast carcinoma with Mammaglobin antibody (clone MGB/4056). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Mammaglobin Antibody - HuProt Validated clone MGB/4056 - protein microarray specificity validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins was performed using Mammaglobin Antibody - HuProt Validated (clone MGB/4056). The strongest binding signal corresponds to SCGB2A2 (Mammaglobin A), which ranks first on the array, demonstrating high specificity of the MGB/4056 mouse monoclonal antibody for its intended target. Z- and S-scores quantify antibody binding strength and target specificity. The Z-score represents the signal intensity produced when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a protein on the array and is expressed as standard deviations above the mean signal of the array. When proteins are ordered by descending Z-score, the S-score represents the difference between adjacent Z-scores and therefore reflects the relative specificity of the antibody for its target protein.