MAML1 Antibody / Mastermind-like protein 1 (FY12765)

Immunofluorescent staining of FFPE human HeLa cells with MAML1 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot analysis of MAML1 using anti-MAML1 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MOLT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAML1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The observed band at ~130 kDa is consistent with the full-length, post-translationally modified form of MAML1 (predicted ~108 kDa), as previously reported for nuclear MAML1 in Notch signaling studies.

Flow cytometry analysis of fixed and permeabilized human Jurkat cells with MAML1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MAML1 antibody.