MAG Antibody / Myelin Associated Glycoprotein Antibody (FY13483)

MAG Antibody Human Cerebral Cortex IHC. Immunohistochemistry was performed on FFPE human cerebral cortex tissue using MAG Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Prominent staining is observed throughout the neuropil and myelinated fiber tracts, with comparatively little neuronal cell body staining, consistent with the localization of Myelin Associated Glycoprotein to oligodendrocyte myelin membranes. These results demonstrate that this Myelin Associated Glycoprotein Antibody is suitable for immunohistochemical detection of endogenous MAG in formalin-fixed, paraffin-embedded human cerebral cortex tissue.

MAG Antibody Human Cerebral Cortex White Matter IHC. Immunohistochemistry was performed on FFPE human cerebral cortex tissue using MAG Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Intense staining is observed throughout the myelinated fiber network within the cortical white matter, while neuronal cell bodies exhibit minimal immunoreactivity, consistent with the localization of Myelin Associated Glycoprotein to oligodendrocyte myelin membranes. These results demonstrate that this Myelin Associated Glycoprotein Antibody is suitable for immunohistochemical detection of endogenous MAG in formalin-fixed, paraffin-embedded human cerebral cortex tissue.

MAG Antibody Human Cerebellar White Matter IHC. Immunohistochemistry was performed on FFPE human cerebellum tissue using MAG Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Strong linear staining is concentrated within the myelinated fibers of the cerebellar white matter, with comparatively weaker staining in the adjacent granular layer, consistent with the localization of Myelin Associated Glycoprotein to oligodendrocyte myelin sheaths. These results demonstrate that this Myelin Associated Glycoprotein Antibody is suitable for immunohistochemical detection of endogenous MAG in formalin-fixed, paraffin-embedded human cerebellum tissue.

MAG Antibody Human Cerebellum IHC. Immunohistochemistry was performed on FFPE human cerebellum tissue using MAG Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Strong immunoreactivity is observed within the myelin-rich white matter, while the cerebellar gray matter exhibits comparatively weaker staining, consistent with the localization of Myelin Associated Glycoprotein to oligodendrocyte myelin sheaths. These results demonstrate that this Myelin Associated Glycoprotein Antibody is suitable for immunohistochemical detection of endogenous MAG in formalin-fixed, paraffin-embedded human cerebellum tissue.

MAG Antibody Mouse Cerebellum IHC. Immunohistochemistry was performed on FFPE mouse cerebellum tissue using MAG Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Strong immunoreactivity is observed within the cerebellar white matter and myelinated fiber tracts, while the surrounding granular layer exhibits comparatively weaker staining, consistent with the localization of Myelin Associated Glycoprotein to oligodendrocyte myelin sheaths. These results demonstrate that this Myelin Associated Glycoprotein Antibody is suitable for immunohistochemical detection of endogenous MAG in formalin-fixed, paraffin-embedded mouse cerebellum tissue.

MAG Antibody Rat Cerebellum IHC. Immunohistochemistry was performed on FFPE rat cerebellum tissue using MAG Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Strong immunoreactivity is observed throughout the myelinated white matter, while the granular layer exhibits comparatively weaker staining, consistent with the localization of Myelin Associated Glycoprotein to oligodendrocyte myelin sheaths and cerebellar fiber tracts. These results demonstrate that this Myelin Associated Glycoprotein Antibody is suitable for immunohistochemical detection of endogenous MAG in formalin-fixed, paraffin-embedded rat cerebellum tissue.

MAG Antibody Rat and Mouse Brain WB. Western blot analysis was performed using MAG Antibody. Lane 1: rat brain tissue lysate; lane 2: mouse brain tissue lysate. A specific immunoreactive band is detected at approximately 100 kDa in both samples, consistent with the heavily glycosylated mature form of Myelin Associated Glycoprotein, which commonly migrates above its predicted molecular weight of approximately 69 kDa. The strong signal in brain tissue reflects the abundant expression of MAG within myelinating oligodendrocytes and myelin-rich regions of the central nervous system. These results demonstrate that this Myelin Associated Glycoprotein Antibody is suitable for Western blot detection of endogenous MAG in rat and mouse brain tissue lysates.