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Home >> Antibodies >> Lysozyme C Antibody / LYZ

Lysozyme C Antibody / LYZ [clone LYZ/3944] (V9673)

  Catalog No Formulation Size Price (USD)  
Image V9673-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9673-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9673SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human spleen tissue and HepG2 cell lysates using Lysozyme C antibody (clone LYZ/3944). Predicted molecular weight ~17 kDa.
IHC staining of FFPE human spleen tissue with Lysozyme C antibody (clone LYZ/3944). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with Lysozyme C antibody (clone LYZ/3944). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Lysozyme C antibody (clone LYZ/3944) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Lysozyme C antibody (clone LYZ/3944). These results demonstrate the foremost specificity of the LYZ/3944 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name LYZ/3944
Purity Protein A/G affinity
UniProt P61626
Localization Secreted
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Lysozyme C antibody is available for research use only.
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Description

Lysozyme is an enzyme, commonly referred to as the body s own antibiotic since it kills bacteria. Natural substrate of lysozyme is the bacterial cell wall peptidoglycan (cleaving the beta[1-4] glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Lysozyme is synthesized predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. This antibody labels myeloid cells, histiocytes, granulocytes, macrophages and monocytes. It is helpful in the identification of myeloid or monocytic nature of acute leukemia.

Application Notes

Optimal dilution of the Lysozyme C antibody should be determined by the researcher.

Immunogen

A portion of amino acids 18-147 was used as the immunogen for the Lysozyme C antibody.

Storage

Aliquot the Lysozyme C antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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