LYPD3 Antibody / Ly6/PLAUR domain-containing protein 3 (RQ8480)

Immunofluorescence analysis of LYPD3 Antibody in human skin cancer tissue. Formalin-fixed, paraffin-embedded human skin cancer sections show strong membranous and peripheral cytoplasmic fluorescence consistent with Ly6/PLAUR domain-containing protein 3 (C4.4A / LYPD3) expression in tumor epithelial cells. LYPD3 antibody signal is visualized in red outlining malignant squamous epithelial cells, while nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming tissue sections in pH 8 EDTA buffer for 20 min prior to antibody incubation.

Immunohistochemistry of LYPD3 Antibody in human skin cancer tissue. Formalin-fixed, paraffin-embedded human skin cancer sections demonstrate membranous HRP-DAB brown staining outlining tumor epithelial cells consistent with Ly6/PLAUR domain-containing protein 3 (C4.4A / LYPD3) expression. Positive staining highlights nests of malignant squamous epithelial cells, while surrounding stromal components show minimal signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 min followed by cooling prior to antibody incubation.

IHC staining of FFPE rat stomach tissue with LYPD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human RT4, 2) human A431, 3) rat stomach and 4) mouse stomach tissue lysate with LYPD3 antibody. Predicted molecular weight ~36 kDa but may be observed at higher molecular weights due to glycosylation.

Flow cytometry testing of fixed and permeabilized human RT4 cells with LYPD3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LYPD3 antibody.