LRRC71 Antibody / Leucine-rich repeat-containing protein 71 (FY12488)

Western blot analysis of C1orf92/LRRC71 using anti-LRRC71 antibody. Lane 1: human PC-3 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC71 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. LRRC71 (~62 kDa predicted) was detected as a major band at ~62 kDa, with a weaker band at ~70 kDa consistent with differential glycosylation or isoform expression. Additional lower bands (~22 kDa) observed in rodent tissues likely represent truncated or species-specific LRRC71 forms, as reported for related LRR-family proteins.