LRRC1 Antibody / Leucine-rich repeat-containing protein 1 (FY12619)

Western blot analysis of LRRC1 using anti-LRRC1 antibody. Lane 1: human Caco-2 whole cell lysates, Lane 2: human whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot probed with anti-LRRC1 shows a major band at the expected ~63 kDa and a faint upper band consistent with a phosphorylated form of LRRC1.