LRIT3 Antibody / Leucine-Rich Repeat Protein Antibody (RQ8551)

LRIT3 Antibody Human U2OS Osteosarcoma Cell IF. Immunofluorescence analysis of human U2OS osteosarcoma cells was performed with LRIT3 Antibody at 5 ug/ml following enzyme antigen retrieval for 15 min. LRIT3 was detected with Cy3-conjugated secondary antibody (red), while beta tubulin was co-stained with DyLight 488-conjugated secondary antibody (green). LRIT3 staining is observed predominantly in the nuclear and perinuclear regions, while beta tubulin highlights the cytoplasmic microtubule network. This LRIT3 Antibody demonstrates cellular localization of the leucine-rich repeat protein in human U2OS cells.

LRIT3 Antibody Human, Mouse and Rat WB. Western blot analysis was performed with LRIT3 Antibody on human 293T and Jurkat cell lysates (lanes 1-2), rat kidney tissue lysate (lane 3) and mouse kidney tissue lysate (lane 4). Samples were separated by 5-20% SDS-PAGE under reducing conditions, and the antibody was used at 0.5 ug/ml overnight at 4oC. A specific band is detected at approximately 75 kDa across the human, rat and mouse samples, consistent with the expected molecular weight of LRIT3. This LRIT3 Antibody demonstrates detection of the leucine-rich repeat protein across multiple species and sample types.