LRH1 Antibody / Nuclear Receptor Transcription Factor Antibody [clone PCRP-NR5A2-1B8] (V4787)

LRH1 Antibody Prostate Carcinoma IHC. Immunohistochemistry staining of FFPE human prostate carcinoma tissue using monoclonal clone PCRP-NR5A2-1B8 demonstrates predominantly nuclear HRP-DAB brown staining within malignant glandular epithelial cells, consistent with localization of Liver receptor homolog 1 / NR5A2 as a nuclear receptor transcription factor involved in metabolic and epithelial regulatory signaling pathways. The staining pattern highlights transcription factor-associated nuclear enrichment within prostate tumor epithelium. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

LRH1 Antibody HeLa FACS. Flow cytometry analysis of PFA-fixed human HeLa cells using monoclonal clone PCRP-NR5A2-1B8 followed by goat anti-mouse IgG-CF488 demonstrates a clear rightward fluorescence shift relative to unstained control cells, supporting intracellular expression of Liver receptor homolog 1 / NR5A2 in cervical carcinoma-derived cells. The observed staining profile is consistent with detection of this metabolic nuclear receptor transcription factor within fixed cellular compartments. Blue=NR5A2 antibody, Red=unstained cells.

LRH1 Antibody HuProt Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using monoclonal clone PCRP-NR5A2-1B8 identified NR5A2 / LRH1 as the highest-ranked target with a Z-score of 39.12 and an S-score of 27.04, supporting strong preferential recognition of this nuclear receptor transcription factor relative to unrelated proteins present on the array. The elevated S-score exceeds the accepted specificity threshold and demonstrates selective target binding by the monoclonal antibody. The Z-score reflects relative signal intensity generated by antibody binding across the proteomic array, while the S-score represents the difference in signal strength between adjacent ranked proteins and serves as a measure of relative target specificity.

SDS-PAGE analysis of purified, BSA-free NR5A2 antibody (clone PCRP-NR5A2-1B8) as confirmation of integrity and purity.