LRG1 Antibody / Angiogenesis Regulation Marker [clone LRG1/4882] (V9588)

LRG1 Antibody Breast Tissue IHC. Immunohistochemistry analysis of FFPE human breast tissue using LRG1 antibody. The mouse monoclonal antibody clone LRG1/4882 shows cytoplasmic staining in glandular epithelial structures, consistent with Leucine-rich alpha-2-glycoprotein 1 / LRG1 expression as an angiogenesis regulation marker. Signal is observed in epithelial and perivascular regions, reflecting the role of this secreted protein in vascular remodeling and tissue microenvironment signaling, while surrounding stromal areas display lower staining. HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling prior to staining.

SDS-PAGE analysis of purified, BSA-free LRG1 antibody (clone LRG1/4882) as confirmation of integrity and purity.

LRG1 Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using LRG1 antibody (clone LRG1/4882). These results demonstrate the foremost specificity of the LRG1/4882 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.