- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
LPXN Antibody / Leupaxin (FY12903)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12903 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
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LPXN antibody detects Leupaxin, an adaptor protein involved in focal adhesion signaling, cytoskeletal organization, and immune cell migration. Encoded by the LPXN gene on chromosome 11q12.1, this protein belongs to the paxillin family of LIM domain-containing adaptors that mediate integrin-dependent signaling and actin cytoskeleton rearrangement. LPXN is primarily expressed in hematopoietic and endothelial cells, where it modulates adhesion dynamics, motility, and signal transduction downstream of integrins and growth factor receptors.
Structurally, Leupaxin is a 395-amino-acid cytoplasmic protein of approximately 46 kilodaltons that contains four C-terminal LIM domains and an N-terminal LD motif region that binds focal adhesion kinase (FAK), PYK2, and other adhesion regulators. These motifs allow LPXN to function as a scaffold linking structural proteins, tyrosine kinases, and cytoskeletal components at focal adhesions. Its phosphorylation on tyrosine residues by FAK and Src family kinases regulates recruitment to adhesion complexes and determines downstream signaling strength.
The LPXN antibody is widely used in cell biology, immunology, and cancer research to study integrin-mediated signaling, immune cell migration, and cytoskeletal regulation. Western blot analysis detects a 46 kilodalton band corresponding to Leupaxin, while immunofluorescence shows punctate cytoplasmic and peripheral staining consistent with focal adhesion localization. This antibody supports studies examining adhesion turnover, cell spreading, and mechanotransduction in immune and endothelial systems.
Functionally, LPXN modulates adhesion strength and migratory capacity in leukocytes and macrophages by coordinating the FAK/PYK2 and paxillin signaling pathways. It influences podosome formation, phagocytosis, and chemotaxis through its ability to regulate actin polymerization and integrin clustering. Dysregulated LPXN expression has been linked to cancer metastasis, inflammatory disorders, and defective immune cell trafficking. The LPXN antibody provides a reliable tool for evaluating these processes and understanding how adhesion adaptors control immune cell behavior. NSJ Bioreagents validates this antibody for western blotting, immunohistochemistry, and immunofluorescence, ensuring dependable performance for adhesion and cytoskeletal studies.
Optimal dilution of the LPXN antibody should be determined by the researcher.
E.coli-derived human LPXN recombinant protein (Position: E45-H323) was used as the immunogen for the LPXN antibody.
After reconstitution, the LPXN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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