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Home >> Antibodies >> LMO2 Antibody / Rhombotin 2

LMO2 Antibody / Rhombotin 2 [clone LMO2/1971] (V7925)

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  Catalog No Formulation Size Price (USD)  
Image V7925-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7925-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7925SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human Ramos cell lysate with LMO2 antibody. Predicted molecular weight ~25 kDa.
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Western blot testing of human Ramos cell lysate with LMO2 antibody. Predicted molecular weight ~25 kDa.
Flow cytometry testing of permeabilized human K562 cells with LMO2 antibody; Red=isotype control, Blue= LMO2 antibody.
IHC staining of FFPE human follicular lymphoma with LMO2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human pancreas with LMO2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free LMO2 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name LMO2/1971
Purity Protein G affinity chromatography
UniProt P25791
Localization Nuclear, cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This LMO2 antibody is available for research use only.
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Description

The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. It has a particular function in normal and lymphatic endothelial cells involving the regulation of angiogenesis and lymph-angiogenesis. Immunohistochemical studies have also demonstrated expression of LMO2 in both normal germinal center B-cells and germinal center-derived B-cell lymphomas, including follicular lymphoma and diffuse large B-cell lymphoma.The use of anti-LMO2 is valuable as a tool in the identification of lymphomas of B-cell origin. LMO2 is useful in differentiating follicular lymphoma (LMO2+) from nodal marginal zone lymphoma (LM02-). It also is positive in Hodgkin s and Burkitt s lymphomas.

Application Notes

Optimal dilution of the LMO2 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 23-140) was used as the immunogen for the LMO2 antibody.

Storage

Store the LMO2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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