LMNB1 Antibody | Nuclear Lamina Protein Antibody

	Catalog No	Formulation	Size	Price (USD)
	R32004	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

LMNB1 Antibody Human Stomach Cancer IHC. Immunohistochemistry staining of FFPE human stomach cancer tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear structure regulation and chromatin-associated cellular pathways.

LMNB1 Antibody Human Urothelial Carcinoma IHC. Immunohistochemistry staining of FFPE human urothelial carcinoma tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, chromatin structure and cellular regulation pathways.

LMNB1 Antibody Human Colorectal Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human colorectal adenocarcinoma tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear architecture and chromatin-associated cellular regulation pathways.

LMNB1 Antibody Human Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear structure maintenance and chromatin-associated cellular regulation pathways.

LMNB1 Antibody Human Glioblastoma IHC. Immunohistochemistry staining of FFPE human glioblastoma tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear architecture and chromatin-associated regulation in cancer biology.

LMNB1 Antibody Mouse Cerebellum IHC. Immunohistochemistry staining of FFPE mouse cerebellum tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, neuronal nuclear structure and chromatin-associated regulation pathways.

LMNB1 Antibody Rat Cerebellum IHC. Immunohistochemistry staining of FFPE rat cerebellum tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, neuronal nuclear architecture and chromatin-associated cellular regulation pathways.

LMNB1 Antibody U-2 OS Cell IF. Immunofluorescent staining of FFPE human U-2 OS cells with LMNB1 Antibody (green). HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes prior to staining. Nuclei were counterstained with DAPI (blue). LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear structure maintenance and chromatin-associated cellular regulation pathways.

LMNB1 Antibody Human, Rat and Mouse WB. Western blot testing of human U-2 OS, THP-1, A431 and RT4 cell lysates, rat brain and lung tissue lysates, and mouse brain and lung tissue lysates with LMNB1 Antibody. Lane 1: human U-2 OS; Lane 2: human THP-1; Lane 3: human A431; Lane 4: human RT4; Lane 5: rat brain; Lane 6: rat lung; Lane 7: mouse brain; Lane 8: mouse lung. A specific band was detected at approximately 66 kDa, consistent with the predicted molecular weight of Lamin B1. LMNB1 Antibody / Nuclear Lamina Protein Antibody detects endogenous LMNB1 expression and supports studies of nuclear lamina organization, nuclear envelope structure and chromatin-associated cellular regulation pathways.

LMNB1 Antibody HeLa Cell IP. Immunoprecipitation of LMNB1 protein from 500 ug of human HeLa whole cell lysate using 2 ug of LMNB1 Antibody. Immunoprecipitated protein was analyzed by Western blot, with input lysate and control IgG samples included for comparison. LMNB1 was successfully enriched and detected at approximately 66 kDa, consistent with the predicted molecular weight of Lamin B1. LMNB1 Antibody / Nuclear Lamina Protein Antibody supports analysis of nuclear lamina protein interactions, nuclear envelope organization and chromatin-associated cellular regulation pathways.

LMNB1 Antibody A431 Cell FACS. Flow cytometry testing of fixed and permeabilized human A431 cells with LMNB1 Antibody at 1 ug/million cells. Cells were blocked with goat serum prior to intracellular staining. Red histogram represents cells alone, green represents isotype control and blue represents LMNB1 antibody staining. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates detection of endogenous Lamin B1 expression and supports studies of nuclear lamina organization, nuclear envelope structure and chromatin-associated cellular regulation pathways.

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LMNB1 Antibody Human Stomach Cancer IHC. Immunohistochemistry staining of FFPE human stomach cancer tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear structure regulation and chromatin-associated cellular pathways.

LMNB1 Antibody Human Urothelial Carcinoma IHC. Immunohistochemistry staining of FFPE human urothelial carcinoma tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, chromatin structure and cellular regulation pathways.

LMNB1 Antibody Human Colorectal Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human colorectal adenocarcinoma tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear architecture and chromatin-associated cellular regulation pathways.

LMNB1 Antibody Human Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear structure maintenance and chromatin-associated cellular regulation pathways.

LMNB1 Antibody Human Glioblastoma IHC. Immunohistochemistry staining of FFPE human glioblastoma tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear architecture and chromatin-associated regulation in cancer biology.

LMNB1 Antibody Mouse Cerebellum IHC. Immunohistochemistry staining of FFPE mouse cerebellum tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, neuronal nuclear structure and chromatin-associated regulation pathways.

LMNB1 Antibody Rat Cerebellum IHC. Immunohistochemistry staining of FFPE rat cerebellum tissue with LMNB1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with LMNB1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, neuronal nuclear architecture and chromatin-associated cellular regulation pathways.

LMNB1 Antibody U-2 OS Cell IF. Immunofluorescent staining of FFPE human U-2 OS cells with LMNB1 Antibody (green). HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes prior to staining. Nuclei were counterstained with DAPI (blue). LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates nuclear envelope staining consistent with Lamin B1 localization and supports studies of nuclear lamina organization, nuclear structure maintenance and chromatin-associated cellular regulation pathways.

LMNB1 Antibody Human, Rat and Mouse WB. Western blot testing of human U-2 OS, THP-1, A431 and RT4 cell lysates, rat brain and lung tissue lysates, and mouse brain and lung tissue lysates with LMNB1 Antibody. Lane 1: human U-2 OS; Lane 2: human THP-1; Lane 3: human A431; Lane 4: human RT4; Lane 5: rat brain; Lane 6: rat lung; Lane 7: mouse brain; Lane 8: mouse lung. A specific band was detected at approximately 66 kDa, consistent with the predicted molecular weight of Lamin B1. LMNB1 Antibody / Nuclear Lamina Protein Antibody detects endogenous LMNB1 expression and supports studies of nuclear lamina organization, nuclear envelope structure and chromatin-associated cellular regulation pathways.

LMNB1 Antibody HeLa Cell IP. Immunoprecipitation of LMNB1 protein from 500 ug of human HeLa whole cell lysate using 2 ug of LMNB1 Antibody. Immunoprecipitated protein was analyzed by Western blot, with input lysate and control IgG samples included for comparison. LMNB1 was successfully enriched and detected at approximately 66 kDa, consistent with the predicted molecular weight of Lamin B1. LMNB1 Antibody / Nuclear Lamina Protein Antibody supports analysis of nuclear lamina protein interactions, nuclear envelope organization and chromatin-associated cellular regulation pathways.

LMNB1 Antibody A431 Cell FACS. Flow cytometry testing of fixed and permeabilized human A431 cells with LMNB1 Antibody at 1 ug/million cells. Cells were blocked with goat serum prior to intracellular staining. Red histogram represents cells alone, green represents isotype control and blue represents LMNB1 antibody staining. LMNB1 Antibody / Nuclear Lamina Protein Antibody demonstrates detection of endogenous Lamin B1 expression and supports studies of nuclear lamina organization, nuclear envelope structure and chromatin-associated cellular regulation pathways.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P20700
Localization	Nuclear membrane, nuclear
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunoprecipitation : 2ug/500ug lysate Immunofluorescence (FFPE) : 2-4ug/ml Flow Cytometry : 1-3ug/million cells
Limitations	This LMNB1 Antibody / Nuclear Lamina Protein Antibody is available for research use only.
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Description

LMNB1 Antibody / Nuclear Lamina Protein Antibody detects Lamin B1, a type V intermediate filament protein and essential structural component of the nuclear lamina. LMNB1 forms a dense filament network beneath the inner nuclear membrane that provides mechanical stability and helps maintain nuclear shape. This nuclear scaffold participates in multiple cellular processes, including chromatin organization, DNA replication, transcriptional regulation and nuclear envelope integrity. Because of its defined localization at the nuclear periphery, Lamin B1 is widely studied as a marker for nuclear lamina structure and nuclear organization.

Lamin B1 contributes to the physical and functional connection between chromatin and the nuclear envelope. Interactions between nuclear lamina proteins and specific chromosomal regions help organize genome positioning and influence gene expression patterns. Proper LMNB1 expression and distribution are important for maintaining nuclear architecture, cellular function and responses to structural stress. LMNB1 Antibody is commonly used by researchers studying nuclear envelope biology, chromatin organization, nuclear morphology and cellular regulation pathways.

Research involving LMNB1 extends into aging, cancer biology, neuroscience and disease-related studies. Changes in Lamin B1 expression have been investigated in cellular senescence models, where alterations in nuclear lamina composition are associated with changes in nuclear organization and gene regulation. Abnormal LMNB1 expression or structure has also been studied in connection with neurological disorders and altered cellular states. These findings highlight the importance of nuclear lamina proteins in maintaining normal cell function and nuclear stability.

LMNB1 Antibody applications include detection of Lamin B1 expression, localization and regulation in biological samples using techniques such as Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation. Analysis of this nuclear lamina protein supports studies of nuclear envelope structure, chromatin organization, cellular senescence and nuclear architecture. NSJ Bioreagents provides LMNB1 Antibody / Nuclear Lamina Protein Antibody for researchers studying nuclear structure, genome organization and cell biology pathways.

LMNB1 Antibody detects a nuclear lamina protein involved in nuclear envelope organization, chromatin structure and cellular senescence pathways; additional nuclear structure research tools are available on our Lamin B1 Antibody / Nuclear Envelope Marker Antibody page.

Application Notes

Optimal dilution of the LMNB1 Antibody / Nuclear Lamina Protein Antibody should be determined by the researcher.

Immunogen

Amino acids 266-583 of human Lamin B1 were used as the immunogen for the LMNB1 antibody.

Storage

After reconstitution, the LMNB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Lamin B1 Antibody, Lamin-B1 Antibody, Nuclear Lamin B1 Antibody, Nuclear Envelope Protein Antibody, Type V Intermediate Filament Antibody, Senescence Marker Antibody

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LMNB1 Antibody / Nuclear Lamina Protein Antibody (R32004)

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Application Notes

Immunogen

Storage

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